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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in several tumors, which ends up in chromosome instability, and so may perhaps add to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two further splice variants were claimed but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind precisely towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified with the antibody.
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This guidebook outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
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This guideline outlines further more feasible optimization of cellular and immunoassay parameters to make website sure the best possible benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining with the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.
Target Data Cyclin E belongs to the hugely conserved cyclin family, whose associates are characterised by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute for the temporal coordination of every mitotic function. Cyclin E kinds a complex with and functions as being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates in the G1-S phase boundary and is also degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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